Expressions and Significances of Prostasin and Claudin-3in ...

[Abstract]

Ovarian cancer is a malignant tumor which has the third most higher incidences in female reproductive system,that only less than the incidences of cervical cancer and endometrial cancer.The mortality rate of ovarian malignant tumor is the first place of gynecologic malignant tumors around the world.Because of lacking potent diagnoses and therapy in the early-stage disease,it is already late that about three of forth ovarian cancer patients were found, and it is just around28%about five-year survival rate. With the disease incidence guadually raises tendency around the world in recent years,looking for the causes?diagnoses and treatment method of ovarian cancer are the hot spots which are concerned by people.However it is not explicit about the pathogenic mechanises of the disease.With the rapid development in molecular biology,medical researchers have found some genes which are related to the occurrence,evolution and invasion and attack of ovarian cancer.Prostasin is a trypsin-like activity serine proteinase,that is synthesized in prostatic epithelial cells and is isolated from human seminal fluid and ductin rencent years.It has highly conserved sequenceis and it is a secretion type protein and glycosylphosphatidyl inositol(GPI)-anchored prostase.Prostasin is not only playing important roles in variety of physiological processes,but also linking to the occurrence and development of varius cancer. It was found that serine proteases was consistent with a role in protease degradation and promoted cells migration.However there are rare researches about the function and expression of Prostasin in ovarian cancer.Claudin-3(CLDN-3) is a number of Claudins,which was called RVP1formerly.It contains219amino acids and provides the foundation of conglutination and the tight junction of maintaining the transmission about signs and matters in the cells.Current researches showed that the abnormal expression of Claudins is probably related to the damage of tight junction,poorly tissue differentiated grade and enhancement of invasiveness about cancer cells,and link to the occurrence and invasiveness of varius cancer. However it is not all clear about the function and mechanism of Claudin-3in ovarian cancer.This study investigated the changes of the expression about Prostasin and Claudin-3and the functions about the happen,developmenthe of epithelial ovarian cance,and analysised relationship of them.The aim is to supply the reference for the diagnoses treatment and prognosis research about ovarian cancer.ObjectiveImmunohistochemical method was used to detect the expression of Prostasin and Claudin-3in epithelial ovarian cancer tissue and normal ovarian tissue in this experiment,we studied the relationship between their expression and various histology pathplogical features in epithelial ovarian cancer,and researched their roles and correlation in of the mechanises of diseasea,in order to provide theory basis for the clinical diagnoses and prognosis of ovarian cancer and to find a new target for treatments of ovarian cancer.Materials and Methods1.Materials:Ninty epithelial oarian tumor tissue specimens were collected from the files organization of the patients who were in hospital and operate in the Third Affiliated Hospital of Zhengzhou University from October2008to February2011,which were from the department of Pathology in that hospital.Specimens is divided into50epithelial ovarian cancers,20borderline ovarian tumors and20benign ovarian tumors.According to histological classification,50epithelial ovarian cancers are divide into three groups and includ27serous carcinoma,15mucinous carcinoma,8endometrial carcinoma.In addition,20cases of normal ovarian tissues were collected as comparator,which were from operation about oophorectomize for uterine prolapse and uterine myoma caused hysterectomy at the same time were taken ovarian tissues for ovarian biopsy.All specimens?pathology diagnosis were confirmed by two pathology experts.The patients?average age was52.2years(36-70years), and they didn?t recept any chemotherapy,radiotherapy,and immune therapy history before their operations.2.Methods:Immunohistochemical SP method was used to detect the expression levels of Prostasin and Claudin-3in50cases with epithelial ovarian carcinoma,20cases with borderline ovarian tumor,20cases with benign ovarian tumor and20cases with normal ovarian tissues,we analyse the relationship between their different expression and each clinical pathological feature in epithelial ovarian cancer,and analyse correlation about Prostasin protein and Claudin-3protein and explore both roles about the occurrence,development and malignant evolution process in epithelial ovanan cancer.3.Statistical Methods:Statistical anylysis was performed using the SPSS17.0statistical software,datas were analyzed by Chi-Square Tests,and their relationship of Prostasin and Claudin-3were analysised by Spearman rank correlation. ?=0.05acted as level of significance.Results1The expressions of ProstasinProstasin protein was mainly displaied in the cytoplasm,and its positive cells were watched for brown dying in the microscope.1.1The expressions of Prostasin in different ovarian tissueThe positive expression lever of Prostasin in epithelial ovarian cancer groups was significantly higher than the expression in borderline groups,benign groups and normal groups,and the overall differences were statistical significances (P<0.05).1.2The expressions of Prostasin in different pathology types of ovarian cancerThe positive expression rates of Prostasin in15G1grade,18G2grade and17G3grade were40%?77.8%and88.2%, and expression differences in three groups have statistical significances (P<0.05)1.3The expressions of Prostasin in different pathology types of ovarian cancerThe expression of Prostasin in serous cystadenoma were highest (77.8%),and the expression in mucinous cystadenoma were lowest(60%). With comparing endometrioid carcinoma,different expression rates between groups had no statistical significances(P>0.05). 1.4The expressions of Prostasin in different clinic stages about ovarian canceThe positive expression level of Prostasin became stronger with the higher stage, the expression rates in21clinic stage ??? and29clinic stage??? were52.4%and82.8%, different expression rates between groups had statistical significances(P<0.05).2The expressions of Claudin-3Claudin-3protein was mainly expressed in the cell membrane and cytoplasm,especially in the cytoplasm,and positive cells were colored brown.2.1The expressions of Claudin-3in different ovarian tissueThe expression rates of Claudin-3was gradually rised in normal groups,benign groups,borderline groups and epithelial ovarian cancer groups, and there were statistical significances in all(P<0.05).2.2The expression of Claudin-3in different pathology types of ovarian cancerThe positive expression rates of Claudin-3in G1grade,G2grade and G3grade were40%?66.7%and82.4%, and differences in three groups had statistical significances (P<0.05)2.3The expressions of Claudin-3in different pathology types of ovarian cancerThe positive expression rates of Claudin-3in epithelial ovarian serous cystadenoma group,mucinous cystadenoma group and endometrioid carcinoma group were74.1%,60.0%and37.5%,groups expression differences had no statistical significance(P>0.05).2.4The expressions of Claudin-3in different clinic stages about ovarian canceThe positive expression level of Claudin-3in clinic stage ??? and ??? were47.6%and75.9%,and with the clinic stage higher,the expression rates became stronger,and there were statistical significances in all (P<0.05)3The relevance of Prostasin and Claudin-3in epithelial ovarian cancerIn50epithelial ovarian cancers,the number of positive expression of Prostasin observed was35,positive expression of Claudin-3was observed in32cases. There was positive correlation between Prostasin and Claudin-3expression in epithelial ovarian cancer (r=0.386, P<0.05).Conclusion1. High expressions of Prostasin and Claudin-3are closely related with the occurrence, development, migration and progression of epithelial ovarian carcinoma.2. There was positive correlation between the expression level of Prostasin and Claudin-3in epithelial ovarian carcinoma,which tiped they may ally to play the important synergistic promote roles in the occurrence,development and progression of ovarian carcer.

Title: Expressions and Significances of Prostasin and Claudin-3in Epithelial Ovarian Carcinoma

Category: Ovarian Cancer

Filename: Expressions and Significances of Prostasin and Claudin-3in Epithelial Ovarian Carcinoma.pdf

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